Protein_Domain

Part:BBa_K2124002:Design

Designed by: Brian Dempsey   Group: iGEM16_Lethbridge_HS   (2016-10-13)


Venom Serine Protease from Cerastes Cerastes - Thrombin analog


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This protein is very small and stabilized by several disulfide bonds. The final construct will be secreted into the periplasmic space with DsbC, a protein that shuffles disfulfide bonds. DsbC will allow the Serine Protease to fold properly with correct disulfide bonding. This will give stable functional protein.


Source

This is from a genomic sequence.

References